|
KMID : 0880220140520070548
|
|
Journal of Microbiology
2014 Volume.52 No. 7 p.548 ~ p.553
|
|
|
Bacillus daqingensis sp. nov., a halophilic, alkaliphilic bacterium isolated fromSaline-sodic soil in Daqing, China
|
|
Wang Shuang
Wang Shuang
Sun Lei
Wei Dan
Zhou Baoku
Zhang Junzheng
Gu Xuejia
Zhang Lei
Liu Ying
Li Yidan
Guo Wei
Jiang Shuang
Pan Yaqing
Wang Yufeng
|
|
|
Abstract
|
|
|
|
An alkaliphilic, moderately halophilic, bacterium, designated strain X10-1T, was isolated from saline-alkaline soil inDaqing, Heilongjiang Province, China. Strain X10-1T was determined to be a Gram-positive aerobe with rod-shaped cells. The isolate was catalase-positive, oxidase-negative, non-motile, and capable of growth at salinities of 0?16% (w/v) NaCl (optimum, 3%). The pHrange for growth was 7.5?11.0 (optimum, pH 10.0). The genomic DNA G+C content was 47.7 mol%. Itsmajor isoprenoid quinone was MK-7 and its cellular fatty acid profile mainly consisted of anteiso-C15:0, anteiso-C17:0, iso-C15:0, C16:0, and iso-C16:0. The peptidoglycan contained meso-diaminopimelic acid as the diagnostic diamino acid. The predominant polar lipids were diphosphatidylglycerol, phosphatidylethanolamine, and phosphatidylglycerol. Phylogenetic analysis based on 16S rRNA gene sequences showed that X10-1T is a member of the genus Bacillus, being most closely related to B. saliphilus DSM15402T (97.8% similarity) and B. agaradhaerens DSM 8721T (96.2%). DNA-DNA relatedness to the type strains of these species was less than 40%. On the basis of the phylogenetic, physiological, and biochemical data, strain X10-1T represents a novel species of the genus Bacillus, for which the name Bacillus daqingensis sp. nov. is proposed. The type strain is X10-1T (=NBRC 109404T = CGMCC 1.12295T).
|
|
|
KEYWORD
|
|
|
Bacillus daqingensis sp. nov., 16S rRNA gene sequence, fatty acid composition, DNA-DNA hybridization
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
  
|
|